Project 1.5: Rapid Hemostasis for the Treatment of Incompressible Wounds

PIs: Olsen, Hammond

 

Uncontrolled bleeding is the largest cause of preventable fatalities in soldiers. While the military has a number of effective treatments for compressible wounds and wounds in extremities such as application of battlefield dressings and pressure or the use of a tourniquet, the treatment of incompressible wounds remains a major challenge. Project 1.5 will develop a set of field-deployable nanomaterials that address hemostasis for warfighter applications based on three complementary technologies. First, fully resorbable sponges coated with biomimetic self-assembling peptides and/or clotting proteins will be developed as highly effective hemostats and anti-infectives to stop rapid blood loss from open wounds. Second, shear-thinning injectable hydrogels based on fibrin-mimetic proteins will be engineered to target deep penetrating wounds. Finally, a systemically injectable hemostat based on nanoparticles crosslinked through biorthogonal click chemistry will be designed to treat wounds where the location of bleeding cannot be reached or cannot be identified. All of the above hemostasis systems will be designed, developed, and tested as a joint collaborative effort with the Army Institute for Surgical Research (AISR). Close collaboration will be facilitated by ISN students working at the AISR for periods up to one semester as research assistants to perform integral parts of their thesis research.

 

 

(a) Negative Staining TEM imaging of self-assembling peptide nanofibers. (b) QCM data showing mass buildup of a RADA16 LbL film. (c) Platelets (green) immunostained for activation marker P-selectin (blue) and coated on collagen.
(d) PEG-b-PLGA nanoparticles imaged via scanning electron microscopy. (e) Schematic of in vitro clot imaging assay.
(f) Images showing different responses in clot structure to various hemostats in the clot imaging assay.